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KMID : 0368419980410010059
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Journal of Plant Biology
1998 Volume.41 No. 1 p.59 ~ p.63
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Characterization of Small GTP-Binding Proteins in Plant Cell
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Jang, Young Joo
Lee, June S./Hur, Kyu C.
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Abstract
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To identify and characterize small GTP-binding proteins in plant cells, GTP-binding studies were performed with electroblotted plant proteins following SDS-polyacrylamide gel electrophoresis using [α-^32P]GTP. Three species of small GTP-binding protein (21, 23 and 27 kD) which have a specific GTP-binding property were identified in the membrane and cytosolic fractions of both monocotyledons (Zea mays) and dicotyledons (Glycine max). Moreover, these three species of small GTP-binding protein were gradually decreased when membranes were treated with hydroxylamine. This result indicates that these small GTP-binding proteins in plant cells are fatty acylated to the membrane lipids. The 27 kDa component was partially purified from hypocotyl membranes of Glycine max, following S-300 gel filtration, phenylsepharose CL-4B, hydroxyapatite, and Q-sepharose column chromatography. This 27 kD protein was found to have both GTP-binding and GTPase activities.
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